Journal: International Journal of Molecular Sciences

Article Title: Exploring the Physical and Biological Aspects of BNCT with a Carboranylmethylbenzo[ b ]acridone Compound in U87 Glioblastoma Cells

doi: 10.3390/ijms232314929

Figure Lengend Snippet: DSB quantification using the ɣ-H2AX assay (up); ( A ) represents the nuclei stained by DAPI, ( B ) the foci, pink dots, identified by TXR. The number of MN per BN cell is also presented for CMBA (down) ( C ). The error bars represent the standard deviation of the three independent experiments. The images were obtained using 64× magnification.

Article Snippet: Then, cells were incubated with an anti-γ-H2AX primary antibody (mouse anti-γ-H2AX (ser139), Stressgen, bioreagents Corp., Canada) at 2 μg/mL for 1 h. After being washed twice with 1% BSA in PBS, cells were incubated with a FITC-conjugated anti-mouse secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at 1 mg/mL for 1 h, followed by three washing steps with PBS.

Techniques: Staining, Standard Deviation